Discrimination of Tsingtaodahua from Other Hop Cultivars and Its Quality Control by Molecular Analysis
Junhua Zhang1, Yan Lin1,2, Yuchao Gu1, Jianjun Dong2 and Wengong Yu1 3
1 Institute of Marine Drug and Foods, Ocean University of China, Qingdao, 266003 China.
2 Research & Development Center, Tsingtao Brewery Co., Ltd., 195A Hongkong East Road, Qingdao, 201600, China.
3 Corresponding author. E-mail: yuwg66@ouc.edu.cn
J. Inst. Brew. 111(2), 229–233, 2005 | VIEW ARTICLE
ABSTRACT
Random amplified polymorphic DNA (RAPD) was used for hop varietal identification, primarily to distinguish Tsingtaodahua, a fine Chinese variety. Eleven typical varieties, including four aroma hops, five bitter hops, Tsingtaodahua and Cluster, were successfully identified on the basis of 28 polymorphic RAPD bands amplified by five random primers. UPGMA analysis of RAPD data showed genetic relationship among analyzed varieties consistent with traditional hop classification. Subsequently, one specific RAPD fragment was converted to a sequence tagged site (STS) marker which can detect as little as a 5% admixture of the variety Kirin 1 in Tsingtaodahua. The RAPD and STS markers can be successfully used for Tsingtaodahua identification and quality control.
Key words:
Discrimination, hop, Humulus lupulus, RAPD, STS marker, Tsingtaodahua, quality control.
Publication no. G-2005-0718-291 ©2005 The Institute & Guild of Brewing