Comparison of Esterase Patterns of Three Yeast Strains As Obtained with Different Synthetic Substrates
Giovanna Lomolino1, Anna Lante1,3, Corrado Paolo Spettoli1 and Andrea Curioni1
1 Dipartimento di Biotecnologie Agrarie, Facoltà di Agraria, Università di Padova, Viale Università 16, 35020 Legnaro (Padova) Italy.
2 Dipartimento Scientifico e Tecnologico, Facoltà di Scienze, Università di Verona, Strada Le Grazie 15, 37134 Verona, Italy.
3 Corresponding author. E-mail: anna.lante@unipd.it
J. Inst. Brew. 111(2), 234–236, 2005 | VIEW ARTICLE
ABSTRACT
The visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity was performed by using different chromogenic and fluorescent substrates. The zymograms revealed the presence of several isoforms in yeast extracts, differing in sub-cellular location, substrate specificity and electrophoretic mobility, indicating a high level of polymorphism.
Key words:
Electrophoretic polymorphism, esterase activity, yeast.
Publication no. G-2005-0718-289 ©2005 The Institute & Guild of Brewing