Determination of Prolamins in Beers by ELISA and SDS-PAGE
P. Kanerva1,2 ,4 , T. Sontag-Strohm3 and P. Lehtonen1
1 Alko Inc., Alcohol Control Laboratory, P.O. Box 279, FIN-01301 Vantaa, Finland.
2 Laboratory of Analytical Chemistry, P.O. Box 55, FIN-00014 University of Helsinki, Finland.
3 Department of Food Technology, P.O. Box 66, FIN-00014 University of Helsinki, Finland.
4 Corresponding author. E-mail: paivi.kanerva@helsinki.fi Pasuunakuja 1 C 26, FIN-00420 Helsinki tel. +358405084064, fax +35897536225
J. Inst. Brew. 111(1), 61-64, 2005 | VIEW ARTICLE
ABSTRACT
Coeliac disease is triggered by exposure to the prolamin protein fraction of wheat, barley, or rye. The prolamin content of five lager beers and one wheat beer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting and seven lager beers and three wheat beers were analyzed by enzyme-linked immunosorbent assay (ELISA). Most of the lager beers were made from barley and some had varying amounts of rice or corn as adjuncts. One of the beers was "gluten-free", having been produced from corn and buckwheat without barley. The lager beer samples were gel-filtered before ELISA or SDS-PAGE analysis. Prolamin proteins were found in all but one beer which was made of corn, rice and barley and which was not the "gluten-free" beer. ELISA analysis was done using a commercially available gluten assay kit. For lager beers, a barley prolamin standard for ELISA was propanolextracted from barley malt instead of using the prolamin standard of the gluten assay kit. As expected, the wheat beers contained much higher amounts of prolamins than the lager beers. The samples were studied by SDS-PAGE to identify different prolamin fractions. Proteins having a relative molecular mass in the range of 8000-17,000 and 38,000 and above were detected in immunoblotting by the prolamin sensitive antibody in the lager beers.
Key words:
Barley, beer, ELISA, immunoblotting, prolamin, SDS-PAGE.
Publication no. G-2005-0318-277 ©2005 The Institute & Guild of Brewing