Lipid Turnover During Inverse Flocculation in Saccharomyces cerevisiae UOFS Y-2330
C.J. Strauss 1, J.L.F. Kock 1,3, B.C. Viljoen 1, P.J. Botes 1, G. Hulse 2 and E. Lodolo 2
1 Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein 9301, South Africa.
2 SABMiller Group Research, 65 Park Lane, Sandown, P.O. Box 782178, Sandton, 2146, South Africa.
3 Corresponding author: E-mail: Kockjl.sci@mail.uovs.ac.za
J. Inst. Brew. 110(3), 207-212, 2004 | VIEW ARTICLE
ABSTRACT
In this study we uncovered that Saccharomyces
cerevisiae UOFS Y-2330 does not only demonstrate
inverse flocculation, but is also characterised
by two different lipid turnover patterns. During
Flo1 phenotype flocculation, this yeast showed two
neutral lipid accumulating stages (i.e. at 8 h and
from 12 h). This is probably triggered by flocculation,
which can be regarded as a survival mechanism where
cells accumulate predominantly neutral lipids as
a reserve energy source - a similar mechanism is
probably operative when cells enter stationary growth.
Contrary to Flo1 behaviour, this strain in NewFlo
phenotype mode demonstrates only a single lipid
accumulation phase i.e. when cells enter stationary
growth, which coincides with increase in flocculation.
In addition, an increase in phospholipids was experienced
during active growth in both flocculation behaviours
i.e. Flo1 and NewFlo probably as a result of active
membrane production.
Key words:
Flo1, flocculation, lipid turnover, NewFlo, Saccharomyces
cerevisiae.
Publication no. G-2004-0811-225 ©2004 The Institute & Guild of Brewing