A CAPS Marker that Distinguishes the Barley Yellow Mosaic Disease Resistance Locus rym1 Derived from Chinese Landrace 'Mokusekko 3'
Y. Okada 1,2, R. Kanatani 1, S. Arai 1 and K. Ito 1
1Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd., Kizaki, Nitta, Gunma 370-0393, Japan
2Corresponding author. E-mail: yoshihiro.okada@sapporobeer.co.jp
J. Inst. Brew. 109(2), 103-105, 2003 | VIEW ARTICLE
ABSTRACT
A Cleaved Amplified Polymorphic Sequences (CAPS) marker has been developed from a restriction fragment length polymorphism (RFLP) marker probe, MWG2134, of barley. This CAPS marker can easily distinguish varieties resistant to Barley Yellow Mosaic disease from susceptible varieties. This suggests that our CAPS marker is useful in distinguishing rym1-carrying varieties from non-carrying ones. The results obtained with this marker were compared with resistance tests performed on infested fields. The molecular assay corresponded well with the resistance tests in all varieties/lines and, therefore, it is helpful in distinguishing rym1 in lines in which other rym genes or quantitative trait loci are present.
Key words:
Barley yellow mosaic disease, CAPS marker, resistant gene, rym1.
Publication no. G-2003-0604-136 ©2003 The Institute & Guild of Brewing