J. Inst. Brew. vol.108:1 (2002) Effect of Growth Media and Strains on Structural Stability in Small Chromosomes (Chromosome I, VI and III) of Bottom-Fermenting Yeast

Effect of Growth Media and Strains on Structural Stability in Small Chromosomes (Chromosome I, VI and III) of Bottom-Fermenting Yeast

Masahide Sato1,2, Junji Watari1 and Masachika Takashio1
1Brewing Research Laboratories, Sapporo Breweries Ltd., 10-Okatohme, Yaizu, Shizuoka, 425-0013 Japan
2Corresponding author. E-mail: Masahide.Sato@sapporobeer.co.jp

J. Inst. Brew. 108(3), 283-285, 2002 | VIEW ARTICLE

ABSTRACT
The PFGE analysis results of 50 clones, each isolated from cultures of two lager strains (P and Q), after passage of 700 generations in wort or YEPD, showed that small chromosomal DNAs (I, VI and III) are susceptible to changes during successive cultivation. These changes may be strain-dependent (P > Q) and the media may also affect the instability in the chromosomal DNAs (YEPD > wort).

Key words:
Bottom-fermenting yeast, genetic instability, PFGE.