Comparison of the GFFF and LALLS Methods for the Measurement of Starch Granule Size Distribution in Spring Barley Caryopses
V. Psota, 1, 5 I. Bohačenko, 2 J. Hartmann, 3 M. Budinská, 4 and J. Chmelík 4
1 The Research Institute of Brewing and Malting, Mostecká 7, 614 00 Brno, Czech Republic
2 Institute of Chemical Technology, Radiová 7, 102 31 Prague, Czech Republic
3 Central Institute for Supervising and Testing in Agriculture, Hroznová 2, 656 06 Brno, Czech Republic
4 Institute of Analytical Chemistry AS CR, Veveoí 97, 611 42 Brno, Czech Republic
5 Corresponding author. E-mail: psota@brno.beerresearch.cz
J. Inst. Brew. 108(2), 200-203, 2002 | VIEW ARTICLE
ABSTRACT
A newly developed method GFFF (Gravitational Field-Flow Fractionation) and the well known method LALLS (Low Angle Laser Light Scattering) were used to assess starch granule size distribution of ten varieties of spring barley. As a distribution criterion, the ratio of starch granule content larger than 8 mm (type A) and smaller than 8
µm (type B) was chosen. Both methods divided the observed set in a similar way. Varieties Akcent, Forum and Atribut formed a variety set with the highest ratio of large and small starch granules. Varieties Scarlet and Kompakt had intermediate ratios. The remaining five varieties Amulet, Novum, Olbram, Tolar and Krona had the lowest ratios of large and small starch granules. Statistical analysis showed that there was a highly significant correlation between the GFFF and LALLS methods.
Key words:
Barley, GFFF, LALLS, starch granule size distribution.
Publication no. G-2002-0611-01R ©2002 The Institute & Guild of Brewing