J. Inst. Brew. vol.108:1 (2002) Flow Cytometric Determinations of Glycogen Content of Yeast During Fermentation

Flow Cytometric Determinations of Glycogen Content of Yeast During Fermentation

K.-J. Hutter ¹
¹ Friedrichstaler Strasse 2b, D-76676 Graben-Neudorf.E-mail: KJHutter@web.de

J. Inst. Brew. 108(1):52-53, 2002 | VIEW ARTICLE

ABSTRACT
Using flow cytometric analysis, the rapid detection of glycogen content of yeast cells in process is possible. Glycogen is a sensitive parameter which can express the physiological state of the yeast cells. A procedure was developed to stain and measure glycogen in yeast cells using flow cytometry. Glycogen content of lager yeast cells was tested after 1 and 15 h of nutrient limitation.

Key words:
Flow cytometry, glycogen, yeast.